Rat astroglial somatomedin/insulin-like growth factor binding proteins: characterization and evidence of biologic function

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Rat astroglial somatomedin/insulin-like growth factor binding proteins: characterization and evidence of biologic function.

Specific binding proteins (BPs) to somatomedin/insulin-like growth factors (Sm/IGFs) have been identified in conditioned media from a variety of cells in culture. By affinity cross-linking using disuccinimidyl suberate, we have covalently cross-linked radiolabeled somatomedin-C/insulin-like growth factor I (Sm-C/IGF I), insulin-like growth factor II (IGF II) and insulin to BPs in conditioned me...

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Characterization of somatomedin/insulin-like growth factor receptors and correlation with biologic action in cultured neonatal rat astroglial cells.

The role of somatomedin/insulin-like growth factors (Sm/IGFs) in neural growth and development is not clearly defined. To characterize Sm/IGF receptors and to correlate binding with the biologic actions of Sm/IGFs in a homogeneous population of neural cells, we isolated and studied a nearly pure population of cultured astroglial monolayers derived from cerebral cortices of neonatal rats. Bindin...

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Isolation and characterization of vitreous insulin-like growth factor binding proteins.

PURPOSE Previous studies from this laboratory revealed that vitreous insulin-like growth factor (IGF) biological activity increases in proliferative diabetic retinopathy and that this activity is normally attenuated by IGFBPs. The goal of this study was to identify and characterize the species involved. METHODS Human and porcine vitreous, plasma, recombinant IGFBP-2, and IGFBP-3 were separate...

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Insulin-like growth factor-binding proteins and bone metabolism.

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ژورنال

عنوان ژورنال: The Journal of Neuroscience

سال: 1988

ISSN: 0270-6474,1529-2401

DOI: 10.1523/jneurosci.08-09-03135.1988